biotechrabbit has recently acquired the cell-free protein expression and RNA synthesis divisions from RiNA GmbH. The former RiNA team of highly skilled experts is now adding more than 20 years of experience in protein and RNA expression to the biotechrabbit R&D team. This combined expertise together with the formerly RiNA R&D and production facilities in Berlin, Adlershof, enable us to offer high level cell-free protein and RNA synthesis services, and the well-established Rapid Translation System.

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BOX_ICONSeptember, 2014 – Applications for cell-free protein synthesis in vesicle based eukaryotic systems



RiNA welcomes recent developments and applications of eukaryotic cell-free protein synthesis systems on the level of membrane proteins that are translocated into natural endogenous membrane vesicles published by the Fraunhofer Institut für Zelltherapie und Immunologie (read the original articles Fenz et al., Sachse et al. a, Sachse et al. b)

RiNA, distributor and itself developer and supplier of pro- and also eukaryotic cell-free protein synthesis systems offers insect cell-based protein synthesis kits with endogenous translationally active membrane vesicles suited for the synthesis of glycosylated proteins and membrane proteins into natural membranes. As an extension to this basic kit RiNA additionally offers a variant for the generation of disulfide-bonded proteins including membrane proteins with disulfide bridges and Fab antibody fragments (read the original article Merk et al).

Besides these specialized insect systems containing membrane vesicles RiNA offers a broad platform of RTS kits  (rapid translation system) based on E. coli and wheat germ allowing not only analytical small scale reactions for the synthesis of for example ambitious enzymes and disulfide bonded proteins such as Fab antibody fragments for screening purposes but also the synthesis of milligrams of protein using dialysis-based kits. A current highlight is the combination of cell-free protein synthesis and Microscale Thermophoresis (MST by NanoTemper Technologies GmbH). By using orthogonal enzymatic labeling RiNA is able to produce site-specifically fluorescence or biotin labeled proteins in high yields allowing the convenient determination of dissociation constants of proteins and their ligands by MST (read the original article e.g. Majkut et al.)

Moreover RiNA offers vectors and template generation systems based on PCR for the direct and rapid application in protein synthesis avoiding tedious cloning procedures. For more information please read here and for numerous applications of the RTS system the RTS reference list.

For detailed information on our publication or a customized offer for RTS Kits, please do not hesitate to contact us.

news_button_announcementBOX_ICONSeptember, 2014 – RiNA combines cell-free Protein Synthesis and Aptamer Generation with in-house Microscale Thermophoresis



RiNA now takes advantage of in-house MicroScale Thermophoresis (MST by NANO TEMPER) Technology for studying interactions of proteins and functional nucleic acid (aptamer) with their ligands on the level of dissociation constants. The measurements are yet offered to customers as part of the Protein Expression Service (PEX). High sensitivity and accuracy of the measurement is achieved by RiNAs technology to provide site-specifically fluorescence labeled proteins in high yields.

In the case of Aptamer Generation (AGS) RiNA uses the MST technology for the determination of dissociation constants in solution in order to realize the natural binding situation of targets and their specific aptamers by avoiding undesired immobilization effects.

For detailed information or a customized offer, please do not hesitate to contact us.


BOX_ICONSeptember, 2014 – Development of a cell-free Wheat Germ based System for  the convenient expression screening of any T7 promoter-based template



Coupled cell-free transcription/translation systems based on Wheat Germ lysate offer a synergetic complementation to bacterial and mammalian based systems. In addition to the general advantages of cell-free systems, Wheat Germ lysate distinguishes itself by a high success rate for the synthesis of eukaryotic proteins applying non-optimized reading frames.

Protein purification is simplified by the absence of bacterial endotoxins, e.g. for application in toxin-sensitive cell-based assays. The success rate for the production of active proteins is enhanced by the optimum reaction temperature of 24°C. Moreover, the compatibility of the system with supplements like detergents and cofactors enables the expression of challenging proteins such as membrane proteins. These advantages are already combined in RiNA’s RTS 100/500 Wheat Germ System that uses the Continuous-Exchange Cell-free (CECF) technology. This technology prolongs the reaction to 24 hours and increases the yield dramatically up to 1 mg per ml reaction.

RiNA has now completed the whole process from screening to preparative protein production by adding a batch system to the above-mentioned well-established CECF-based systems. This new screening system allows the rapid protein expression within 2.5 hours. In addition to established cell-free wheat germ vectors users take advantage of the acceptance a very broad range of templates of pro- and eukaryotic origin containing a phage T7 transcription promoter. These templates are often already available in laboratories in large numbers or can be easily ordered at facilities providing large clone libraries. By this approach, multiple constructs – including non-wheat germ-optimized vectors – can be screened rapidly for expression and functionality in parallel saving precious time otherwise required for template generation, e.g. by tedious subcloning procedures. The new batch system yields up to 100 μg protein per ml reaction that is sufficient for many applications.

This Wheat Germ batch system will be offered soon as an easy-to-handle kit to be used with standard reaction tubes and regular laboratory equipment.

For detailed information or a customized offer, please do not hesitate to contact us.


BOX_ICONAugust, 2014 – RiNA involved in foundation of “International Society on Aptamers”



The successful 1st Oxford Symposium on Aptamers “Aptamer 2014” happened on two days in Oxford, UK. The scientific lectures, poster presentations and industrial exhibition stands have been visited by over hundred scientists and students. The symposium has focused on therapeutic, diagnostic, analytical as well as basic research applications of aptamers.

The foundation of “International Society on Aptamers” has established in the context of the symposium. The main ambition of the society is bringing together the academic and industrial aptamer researchers and the solution providers. RiNA represented by Dr. Marcus Menger, director of the Functional Nucleic Acids Division of RiNA, will support actively the society as director in the executive committee.

For detailed information or a customized offer, please do not hesitate to contact us.